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    GFP tag
    商家編碼:66002-1-Ig
    市 場 價:-
    售      價:980.00 請登錄,確認是否享受優惠
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    980.00 庫存:100
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    廠家: proteintech
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    Species specificity:
    recombinant protein, human

    Positive WB detected in:
    Recombinant Protein

    Positive IP detected in:
    Transfected HEK-293 cells

    Positive IF detected in:
    Transfected HEK-293 cells

    Recommended dilution:
    WB : 1:2000-1:16000
    IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
    IF : 1:20-1:200

    Product Information


    Source:
    Mouse

    Purification method:
    Protein A purification

    Isotype:
    IgG2a

    Storage:
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

    Immunogen Information


    Full name:
    GFP tag

    Calculated molecular weight:
    26 kDa

    Observed molecular weight:

    GenBank accession number:

    Gene ID (NCBI):

    Gene symbol

    Synonyms
    CFP, eGFP, eYFP, GFP, GFP tag, YFP
    Background

    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYFP tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.


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